Modeling Leakage Kinetics from Multilamellar Vesicles for Membrane Permeability Determination: Application to Glucose
نویسندگان
چکیده
منابع مشابه
Modeling leakage kinetics from multilamellar vesicles for membrane permeability determination: application to glucose.
The glucose permeability of bilayers formed from phosphatidylcholine, Brij30, and sodium octadecyl sulfate has been determined via an enzymatic reaction. Glucose is encapsulated in either uni- or multilamellar vesicles (MLV) and its concentration in the dispersion medium is monitored by spectrophotometry analysis through the rate of glucose oxidase-catalyzed reaction of glucose oxidation. A kin...
متن کاملDeformability of multilamellar vesicles.
Although a free unilamellar vesicle has zero or almost zero genuine surface tension, the multilamellar vesicle ("onion") exhibits a nonzero effective surface tension sigma(eff). The expression for sigma(eff) used in the literature is sigma(eff) approximately square root of kappaB/d(0), where B is the interaction modulus between the vesicle bilayers, d(0) the repeating distance between the bilay...
متن کاملSmall-angle scattering model for multilamellar vesicles.
A small-angle neutron-x-ray-light-scattering model for multilamellar vesicles is developed on the basis of a simple geometry. N spherical shells with radii of an arithmetic series are allowed for displacements DeltaR which are limited by DeltaR<R/N due to sterical reasons, with R being the radius of the vesicle. The model shows many properties over a large Q range which include a Guinier region...
متن کاملGlucose transport in plasma membrane vesicles from rat adipose tissue.
Plasma membranes of a high degree of purity have been prepared from isolated adipose cells of rat epididymal fat pads. r+H]Glucose uptake by the membranes has been measured with a Millipore filtration technique, with L-[I%]glucose serving as a marker for nonspecific uptake or trapping of the sugars. The plasma membrane preparation shows glucose transport as evidenced by the following: (a) n-glu...
متن کاملMultilamellar Vesicles in a Commercial Surfactant System
The manufacture of concentrated detergent granules in the detergent industry can be achie®ed by the incorporation of surfactant pastes, such as linear alkylbenzene sulfonate ( ) ( paste LAS a lamellar liquid crystalline material surfactant concentration of ca. 78 ) wt. % . LAS paste was processed at 25 C and 60 C, in both the absence and presence ( ) of sodium disilicate Na O:2SiO additi®e, and...
متن کاملذخیره در منابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
ژورنال
عنوان ژورنال: Biophysical Journal
سال: 2006
ISSN: 0006-3495
DOI: 10.1529/biophysj.106.088401